Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite lay...

Development of a novel reagentless, screen– printed amperometric biosensor based on glutamate dehydrogenase and NAD

Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food.

A monosodium glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has been developed and studied for analysis of MSG in sauces, soup etc. The immobilized enzymatic membrane was attached with oxygen electrode with a push cap system. The detection limit of the sensor was 1 mg/dl and the standard curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2 min. ...

Development of a Disposable Screen Printed Amperometric Biosensor Based on Glutamate Dehydrogenase, for the Determination of Glutamate in Clinical and Food Applications

A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola’s Blue (MB) has been investigated as the base transducer for a glutamate biosensor. The sandwich biosensor was fabricated by firstly depositing a chitosan (CHIT) layer onto the surface of the transducer (MB-SPCE), followed by glutamate dehydrogenase (GLDH): this device is designated GLDH-CHIT-MB-SPCE. NAD was added ...

Amperometric measurement of glutamate

ii Abstract (in Finnish) iiiin Finnish) iii